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2 edition of Analysis of the major cellulase of Erwinia carotovora found in the catalog.

Analysis of the major cellulase of Erwinia carotovora

Victoria Joyce Courtice Cooper

Analysis of the major cellulase of Erwinia carotovora

by Victoria Joyce Courtice Cooper

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Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (M.Sc.) - University of Warwick, 1992.

StatementVictoria Joyce Courtice Cooper.
ID Numbers
Open LibraryOL21279592M

The structural gene for the major cellulase of Erwinia carotovora subspecies carotovora (Ecc) was isolated and expressed in Escherichia coli. Sequencing of the gene (celV) revealed a typical signal sequence and two functional domains in the enzyme; a catalytic domain linked by a short proline/threonine-rich linker to a cellulose-binding domain. Cellulase is an important virulence determinant in Erwinia carotovora ssp. carotovora (44), but the role of protease in virulence is ill‐defined (11 ; 37). The synthesis of these different exoenzymes is co‐ordinately regulated by a complex, but ill‐understood, interaction of a variety of gene by:

The enterobacterium Erwinia carotovora ssp. carotovora strain 71 (hereafter Ecc71) produces extracellular enzymes such as pectate lyase isozymes (Pels), cellulase (Cel), polygalacturonase (Peh. Erwinia carotovora subsp. carotovora (Jones) Bergey et al. is a phytopathogenic enterobacterium responsible for the soft rot, blackleg, or stem rot of a number of economically important crops. The disease is characterized by extensive maceration of the affected tissue caused by a variety of plant cell wall-degrading enzymes secreted by the by:

Genetic resistance currently present in cultivars is insufficient to protect the crop. New sources of polygenic resistance to Erwinia carotovora ssp. atroseptica (van Hall) Dye (Eca) have been selected in diploid hybrids originating from intercrossing Solanum tuberosum L with the wild species S. chacoense Bitter and S. yungasense Hawk. Erwinia carotovora is the main bacterial disease of cyclamen.. The symptoms of its onset are a sudden rotting of the tuber, which gives off a fetidsmell, and a drooping of the leaves. The bacterium is present in most soils, but it is fostered by too much warmth, too much fertiliser, and the use of too heavy a growing medium which makes for too much humidity.


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Analysis of the major cellulase of Erwinia carotovora by Victoria Joyce Courtice Cooper Download PDF EPUB FB2

The structural gene for the major cellulase of Erwinia carotovora subspecies carotovora (Ecc) was isolated and expressed in Escherichia coli. Sequencing of the gene (celV) revealed a typical signal sequence and two functional domains in the enzyme; a catalytic domain linked by a short proline/threonine-rich linker to a cellulose-binding domain (CBD).Cited by: Erwinia carotovora, Erwinia chrysanthemi, and Erwinia rhapontici are heterogeneous, but distinct from each other and from Analysis of the major cellulase of Erwinia carotovora book other phena.

The type strains of Erwinia herbicola, Enterobacter agglomerans, and Erwinia milletiae fall into one phenon, and strains of Erwinia ananas and Erwinia Cited by: Erwinia species, Erwinia carotovora subspecies carotovora (Ecc), Erwinia carotovora subspecies atroseptica (Eca), Erwinia chrysanthemi (Echr), and Erwinia amylovora (Ea), are phytopathogens and regulate the production of carbapenem antibiotic and exoenzymes, such as pectate lyases, cellulase, and proteases, through quorum sensing.

– Each strain normally synthesizes one or two major. Results and Discussion Cellulase is detectable in the growth medium of Rhizobium spp. containing a cloned cellulase gene from Erwinia pHKC1 carries a cellulase gene from Erwinia carotovora subsp, carotovora strain Ecc71 on a Fig. Expression of a cellulase promoter during by: 7.

IN Northern Ireland black leg and tuber rot of the potato are caused by Erwinia carotovora variety `Atroseptica' (van Hall) Holland, but two other soft rot coliforms, namely, E. carotovora (Jones. Erwinia carotovora bacterium for biological control of soft rot.

Material and Methods Potato Tuber as a Source of Pathogens The potato tubers of Picasso, Sante, and Nevskiy varieties were used for direct isolation of E. carotovora ssp.

carotovora (Ecc) (Table 1). Infected samples of potatoes showing the characteristic symptoms of soft File Size: KB. Erwinia carotovora infection, [b] Erwinia carotovora subsp. atroseptica -Black Leg (Soft Rot) of Potato, [c] Lesions associated with lenticels on a potato tuber caused either by Erwinia carotovora pv.

atroseptica, or Erwinia carotovora pv. carotvora. world food crops (FAOSTAT, ) and also one of the important food crops in India. Members of two genera of Gram‐negative bacteria, Serratia and Erwinia, produce a β‐lactam antibiotic, 1‐carbapen‐2‐em‐3‐carboxylic have reported previously the cloning and sequencing of the genes responsible for production of this carbapenem in Erwinia genes are organized as an operon, carA–H, and are controlled by a LuxR‐type transcriptional.

Comparative analysis of the five major Erwinia chrysanthemi pectate lyases: enzyme characteristics and potential inhibitors. F Tardy, W Nasser, J Robert-Baudouy, and N Hugouvieux-Cotte-Pattat Laboratoire de Génétique Moléculaire des Microorganismes, UMR Cited by: The complete nucleotide sequence of the cellulase gene was determined and shown to encode a protein of amino acids which would have a predicted molecular weight of  kDa.

The sequence shows a leader sequence for secretion and two typical cellulase domains (a catalytic domain and a cellulose binding domain).Cited by: CelV is the major secreted beta-1,4-endoglucanase of the phytopathogen Erwinia carotovora subsp.

carotovora. Its role in virulence was investigated by comparing the ability of a cellulase-deficient mutant to cause soft rot of potato tubers with that of the wild type. A significant role was demonstrated despite the inability of CelV to effect soft rot when expressed in Escherichia coli. Erwinia carotovora ssp.

carotovora (Ecc) was isolated from commercial pepper (Capsicum annuum) seed lots and identified according to biochemical and pathogenicity tests, cellular fatty acid composition, and PCR reaction with primers based on the pel gene sequence.

RAPD-PCR analysis using nine arbitrary primers showed similarity betweenCited by: The major secreted cellulase (Ce1V) of Erwinia carotovora is an important soft rot virulence factor Mol Plant-Microbe Interact ;– CrossRef Google Scholar 9.

Murata H, McEvoy JL, Chatterjee A, Collmer, A, Chatterjee by: 6. The Erwinia chrysanthemi (strain ) celY gene encoding the minor endoglucanase (EGY) was sequenced.

The analysis of the upstream region allowed us to identify an in vivo active promoter recognized by the NtrA (σ 54) similarity was found between the predicted amino acid (aa) sequences of EGY and either the Er.

chrysanthemi major endoglucanase, EGZ, or the Er. carotovora Cited by:   The ceIV1 gene encoding a secreted cellulase (CelV1) of Erwinia carotovora subsp. carotovora SCC was cloned and its nucleotide sequence determined.

The gene contains an open reading frame of by and codes for an exported protein of amino by: Dendrogram analysis allowed to point out that E. carotovora is close to the genus Serratia. After reconstitution into planar lipid bilayers, this major protein induced ion channels with a major conductance level of pS in 1 M NaCl and a weak cationic by: 4.

Most members of the Erwinia chrysanthemi group are secreting hydrolytic enzymes such as pectinases, proteases, cellulases and phospholipase (Starr and Chatterjee). Soft-rot disease and plant tissue maceration probably result from concerted activity of some or all of these enzymes.

The phytopathogenic bacteria Erwinia carotovora infects Drosophilaand activates an Northern blot analysis, and bacterial counts. For bacterial E. carotovora (Ec) Other Erwinia (E) E amylovora* 1 E amylovora* 2 Ec carotovora 15* E amylovora* 1Cited by: INTRODUCTION.

Erwinia carotovora is a Gram‐negative, enteric phytopathogen that is an important cause of soft rot disease, including tuber and stem rot in carotovora causes tissue maceration by the production of secreted plant cell‐wall‐degrading enzymes (including pectinases, cellulases and proteases), with secretion of large amounts of pectinase enzymes (including pectate Cited by:   Summary.

The aim of this work was to physiologically characterize Erwinia chrysanthemi (strain ) cellulase (EGZ) secretion as a prelude to a more molecular analysis. First, we showed that EGZ secretion occurs in the late stage of logarithmic growth phase while no intracellular pool could be detected by by:.

Erwinia carotovora virulence factors (n ' Q 0 cn V a U _# 0 o 0 L 0 10 5 0 0' 0 1.Eleven strains of Erwinia carotovora that were isolated mainly, but not exclusively, from slimy rot of witloof chicory and were previously designated 'atypical' E. carotovora subsp. atroseptica. Drosophila melanogaster is a natural vector for Erwinia carotovora atroseptica and Erwinia carotovora carotovora, which cause potato blackleg disease.

Drosophila carrying these species are found in potato fields, and at least under greenhouse conditions, Drosophila can spread blackleg disease among potato plants (6, 7).